![]() ![]() Comparison by scaffold composition indicated increased mRNA expressions of hyaline cartilage-associated collagen II, aggrecan, and SOX9 in collagen scaffolds, although expression of collagen X, which is related to hypertrophic cartilage, was also elevated ( P <0.05). GAG/DNA was augmented with hypoxic isolation/expansion in all constructs ( P <0.01). Collagen and HA scaffolds seeded with BMSCs that were isolated, expanded, and differentiated under hypoxia exhibited superior aggrecan and collagen II mRNA expressions ( P <0.05), GAG quantity ( P <0.05), and proteoglycan staining in comparison with normoxia. Isolation/expansion under hypoxia resulted in faster BMSC population doublings per day ( P <0.05), whereas cell and colony counts were not significantly different ( Pā=ā0.60 and 0.30, respectively). Cultured constructs were assessed for gene expression, proteoglycan staining, glycosaminoglycan (GAG) quantity, and diameter change. ![]() Chondrogenic differentiation was performed in a defined medium under hypoxia or normoxia for 14 days. BMSCs were seeded at 10 million cells per cubic centimeter on cylindrical scaffolds composed of either collagen I sponge or esterified HA non-woven mesh. Cell proliferation and colony-forming characteristics were assessed. Ovine BMSCs were isolated and expanded to passage 2 under hypoxia (3% oxygen) or normoxia (21% oxygen). ![]() It was hypothesized that hypoxic isolation/expansion and differentiation would improve BMSC chondrogenesis in each construct. The objective of this study was to assess the effect of hypoxia on in vitro BMSC chondrogenesis within clinically approved porous scaffolds composed of collagen and hyaluronic acid (HA). Therefore, culture conditions capable of modulating tissue phenotype, such as oxygen tension and scaffold composition, are under investigation. The quality of cartilaginous tissue derived from bone marrow mesenchymal stromal stem cell (BMSC) transplantation has been correlated with clinical outcome. ![]()
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